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rd cells  (Vector Laboratories)


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    Structured Review

    Vector Laboratories rd cells
    Rd Cells, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/FL-1301-2/Fluorescein+labeled+Sambucus+Nigra+Lectin+(SNA%2C+EBL)/pm42037410-272-0-18
    Average 95 stars, based on 170 article reviews
    rd cells - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: Recapitulating whipworm development in vitro using caecaloids
    Article Snippet: Stains were then washed in PBS with 0.05% Tween (Sigma-Aldrich) three times, and individual worms were transferred by wide bore pipette tip or directly to slides, micro-manipulated to separate individuals using fine probes or a p20 pipette, and mounted in Fluoromount G (Invitrogen Thermo Fisher Scientific).

    Labeling:

    Article Title: The growth and pathogenesis of Citrobacter rodentium are compromised when multiple mucin sugar utilization pathways are disrupted, leading to accumulation of N-acetylglucosamine 6-phosphate
    Article Snippet: .. Paraffin-embedded colonic tissue sections (5 μm) were deparaffinized by heating at 60°C for 15 min, cleared with xylene, and rehydrated with 100%, 95%, and 70% ethanol, followed by dH 2 O. Dewaxed and dehydrated colonic tissue sections were blocked with Donkey Serum buffer at room temperature (RT) for 1 h. Fluorescently labeled lectins were diluted in antibody dilution buffer and used for staining: WGA (Catalog #FL-1021, Vector Laboratories, 1:500) for GlcNAc, and SNA (Catalog #FL-1301, Vector Laboratories, 1:200) for NeuNAc. ..

    Article Title: The growth and pathogenesis of Citrobacter rodentium are compromised when multiple mucin sugar utilization pathways are disrupted, leading to accumulation of N-acetylglucosamine 6-phosphate.
    Article Snippet: .. Donkey Serum buffer at room temperature (RT) for 1 h. Fluorescently labeled lectins were diluted in antibody dilution buffer and used for staining: WGA (Catalog #FL-1021, Vector Laboratories, 1:500) for GlcNAc, and SNA (Catalog #FL-1301, Vector Laboratories, 1:200) for NeuNAc. ..

    Article Title: An atlas of human siglecs integrates expression, affinity, and cis/trans sialoglycan recognition profiles.
    Article Snippet: .. FITC-PNA (FL-1071) and FITC-SNA (FL-1301) were purchase AR TI CL E IN P RE SS from Vector Laboratories Inc. and were used 1:1000 (V:V) in Hank’s Balanced Salt Solution (HBSS). diCBM40 was produced in house and labeled with AF647-NHS and was used at used 1:1000 (V:V) HBSS. ..

    Staining:

    Article Title: The growth and pathogenesis of Citrobacter rodentium are compromised when multiple mucin sugar utilization pathways are disrupted, leading to accumulation of N-acetylglucosamine 6-phosphate
    Article Snippet: .. Paraffin-embedded colonic tissue sections (5 μm) were deparaffinized by heating at 60°C for 15 min, cleared with xylene, and rehydrated with 100%, 95%, and 70% ethanol, followed by dH 2 O. Dewaxed and dehydrated colonic tissue sections were blocked with Donkey Serum buffer at room temperature (RT) for 1 h. Fluorescently labeled lectins were diluted in antibody dilution buffer and used for staining: WGA (Catalog #FL-1021, Vector Laboratories, 1:500) for GlcNAc, and SNA (Catalog #FL-1301, Vector Laboratories, 1:200) for NeuNAc. ..

    Article Title: The growth and pathogenesis of Citrobacter rodentium are compromised when multiple mucin sugar utilization pathways are disrupted, leading to accumulation of N-acetylglucosamine 6-phosphate.
    Article Snippet: .. Donkey Serum buffer at room temperature (RT) for 1 h. Fluorescently labeled lectins were diluted in antibody dilution buffer and used for staining: WGA (Catalog #FL-1021, Vector Laboratories, 1:500) for GlcNAc, and SNA (Catalog #FL-1301, Vector Laboratories, 1:200) for NeuNAc. ..

    Whole Genome Amplification:

    Article Title: The growth and pathogenesis of Citrobacter rodentium are compromised when multiple mucin sugar utilization pathways are disrupted, leading to accumulation of N-acetylglucosamine 6-phosphate
    Article Snippet: .. Paraffin-embedded colonic tissue sections (5 μm) were deparaffinized by heating at 60°C for 15 min, cleared with xylene, and rehydrated with 100%, 95%, and 70% ethanol, followed by dH 2 O. Dewaxed and dehydrated colonic tissue sections were blocked with Donkey Serum buffer at room temperature (RT) for 1 h. Fluorescently labeled lectins were diluted in antibody dilution buffer and used for staining: WGA (Catalog #FL-1021, Vector Laboratories, 1:500) for GlcNAc, and SNA (Catalog #FL-1301, Vector Laboratories, 1:200) for NeuNAc. ..

    Article Title: The growth and pathogenesis of Citrobacter rodentium are compromised when multiple mucin sugar utilization pathways are disrupted, leading to accumulation of N-acetylglucosamine 6-phosphate.
    Article Snippet: .. Donkey Serum buffer at room temperature (RT) for 1 h. Fluorescently labeled lectins were diluted in antibody dilution buffer and used for staining: WGA (Catalog #FL-1021, Vector Laboratories, 1:500) for GlcNAc, and SNA (Catalog #FL-1301, Vector Laboratories, 1:200) for NeuNAc. ..

    Produced:

    Article Title: An atlas of human siglecs integrates expression, affinity, and cis/trans sialoglycan recognition profiles.
    Article Snippet: .. FITC-PNA (FL-1071) and FITC-SNA (FL-1301) were purchase AR TI CL E IN P RE SS from Vector Laboratories Inc. and were used 1:1000 (V:V) in Hank’s Balanced Salt Solution (HBSS). diCBM40 was produced in house and labeled with AF647-NHS and was used at used 1:1000 (V:V) HBSS. ..

    Concentration Assay:

    Article Title: High avidity phase-separated RNA-protein sialogranules sense lectins and inhibit influenza infection
    Article Snippet: .. 2mg/ml stock concentration of FITC-labelled SNA Lectin (Vector Labs, cat. FL-1301-2) was serially diluted 2-fold nine times in biological water. .. 2mg/ml stock concentration of FITC-labelled SNA Lectin (Vector Labs, cat. FL-1301-2) was serially diluted 2-fold nine times in biological water.



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    C. rodentium utilizes GlcNAc and NeuNAc that are enriched in the murine colon of C57BL/6J mice. ( A ) Growth assay of WT C. rodentium in minimal media with five monosaccharides constituting the Muc2 O-glycans. The absorbance at OD 600 was measured every hour and was shown as mean ± SEM from biological triplicates. ( B ) Wheat germ agglutinin (WGA) and ( C ) Sambucus nigra agglutinin <t>(SNA)</t> <t>staining</t> of colonic cross sections from uninfected mice (at baseline). Sections were stained with WGA or SNA (green) to detect GlcNAc or NeuNAc, respectively, and 4′,6′-diamidino-2-phenylindole (DAPI) (blue) to detect DNA. Original magnification, 200×. Images are representative of three independent experiments with five mice per experiment (scale bar, 20 μm). ( D ) Levels of GlcNAc and NeuNAc in the stools of Muc2 +/+ and Muc2 −/− littermates assessed by an ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC/QqQ-MS). Data were collected from 10 mice pooled from two independent experiments and shown as mean ± SEM. Statistical significance was determined by multiple Mann-Whitney tests. **** P < 0.0001, *** P < 0.001. Levels of ( E ) GlcNAc and ( F ) NeuNAc in the stool samples of mice at baseline and during infection with C. rodentium were assessed by UHPLC/QqQ-MS. Data were collected from eight mice pooled from three independent experiments and shown as mean ± SEM. Statistical significance was determined by Kruskal-Wallis tests (E and F). * P < 0.05. The Y-axis represents the amount of sugar in micrograms per gram of stool ( D–F ).
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    Image Search Results


    C. rodentium utilizes GlcNAc and NeuNAc that are enriched in the murine colon of C57BL/6J mice. ( A ) Growth assay of WT C. rodentium in minimal media with five monosaccharides constituting the Muc2 O-glycans. The absorbance at OD 600 was measured every hour and was shown as mean ± SEM from biological triplicates. ( B ) Wheat germ agglutinin (WGA) and ( C ) Sambucus nigra agglutinin (SNA) staining of colonic cross sections from uninfected mice (at baseline). Sections were stained with WGA or SNA (green) to detect GlcNAc or NeuNAc, respectively, and 4′,6′-diamidino-2-phenylindole (DAPI) (blue) to detect DNA. Original magnification, 200×. Images are representative of three independent experiments with five mice per experiment (scale bar, 20 μm). ( D ) Levels of GlcNAc and NeuNAc in the stools of Muc2 +/+ and Muc2 −/− littermates assessed by an ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC/QqQ-MS). Data were collected from 10 mice pooled from two independent experiments and shown as mean ± SEM. Statistical significance was determined by multiple Mann-Whitney tests. **** P < 0.0001, *** P < 0.001. Levels of ( E ) GlcNAc and ( F ) NeuNAc in the stool samples of mice at baseline and during infection with C. rodentium were assessed by UHPLC/QqQ-MS. Data were collected from eight mice pooled from three independent experiments and shown as mean ± SEM. Statistical significance was determined by Kruskal-Wallis tests (E and F). * P < 0.05. The Y-axis represents the amount of sugar in micrograms per gram of stool ( D–F ).

    Journal: Infection and Immunity

    Article Title: The growth and pathogenesis of Citrobacter rodentium are compromised when multiple mucin sugar utilization pathways are disrupted, leading to accumulation of N-acetylglucosamine 6-phosphate

    doi: 10.1128/iai.00545-25

    Figure Lengend Snippet: C. rodentium utilizes GlcNAc and NeuNAc that are enriched in the murine colon of C57BL/6J mice. ( A ) Growth assay of WT C. rodentium in minimal media with five monosaccharides constituting the Muc2 O-glycans. The absorbance at OD 600 was measured every hour and was shown as mean ± SEM from biological triplicates. ( B ) Wheat germ agglutinin (WGA) and ( C ) Sambucus nigra agglutinin (SNA) staining of colonic cross sections from uninfected mice (at baseline). Sections were stained with WGA or SNA (green) to detect GlcNAc or NeuNAc, respectively, and 4′,6′-diamidino-2-phenylindole (DAPI) (blue) to detect DNA. Original magnification, 200×. Images are representative of three independent experiments with five mice per experiment (scale bar, 20 μm). ( D ) Levels of GlcNAc and NeuNAc in the stools of Muc2 +/+ and Muc2 −/− littermates assessed by an ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC/QqQ-MS). Data were collected from 10 mice pooled from two independent experiments and shown as mean ± SEM. Statistical significance was determined by multiple Mann-Whitney tests. **** P < 0.0001, *** P < 0.001. Levels of ( E ) GlcNAc and ( F ) NeuNAc in the stool samples of mice at baseline and during infection with C. rodentium were assessed by UHPLC/QqQ-MS. Data were collected from eight mice pooled from three independent experiments and shown as mean ± SEM. Statistical significance was determined by Kruskal-Wallis tests (E and F). * P < 0.05. The Y-axis represents the amount of sugar in micrograms per gram of stool ( D–F ).

    Article Snippet: Paraffin-embedded colonic tissue sections (5 μm) were deparaffinized by heating at 60°C for 15 min, cleared with xylene, and rehydrated with 100%, 95%, and 70% ethanol, followed by dH 2 O. Dewaxed and dehydrated colonic tissue sections were blocked with Donkey Serum buffer at room temperature (RT) for 1 h. Fluorescently labeled lectins were diluted in antibody dilution buffer and used for staining: WGA (Catalog #FL-1021, Vector Laboratories, 1:500) for GlcNAc, and SNA (Catalog #FL-1301, Vector Laboratories, 1:200) for NeuNAc.

    Techniques: Growth Assay, Staining, High Performance Liquid Chromatography, Targeted Proteomics, Mass Spectrometry, MANN-WHITNEY, Infection